Deep Sequencing Reveals Complex Spurious Transcription from Transiently Transfected Plasmids

Transient plasmid transfection is a common approach in studies in cultured mammalian cells. To examine behavior of transfected plasmids, we analyzed their transcriptional landscape by deep sequencing. We have found that the entire plasmid sequence is transcribed at different levels. Spurious transcr...

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Detalles Bibliográficos
Autores principales: Nejepinska, Jana, Malik, Radek, Moravec, Martin, Svoboda, Petr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3420890/
https://www.ncbi.nlm.nih.gov/pubmed/22916237
http://dx.doi.org/10.1371/journal.pone.0043283

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3420890/
https://www.ncbi.nlm.nih.gov/pubmed/22916237
http://dx.doi.org/10.1371/journal.pone.0043283

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