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Deep Sequencing Reveals Complex Spurious Transcription from Transiently Transfected Plasmids
Transient plasmid transfection is a common approach in studies in cultured mammalian cells. To examine behavior of transfected plasmids, we analyzed their transcriptional landscape by deep sequencing. We have found that the entire plasmid sequence is transcribed at different levels. Spurious transcr...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3420890/ https://www.ncbi.nlm.nih.gov/pubmed/22916237 http://dx.doi.org/10.1371/journal.pone.0043283 |