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Accurate Estimation of Nucleic Acids by Amplification Efficiency Dependent PCR
Accurate estimation of template - DNA or RNA by real time PCR is dependent on the amplification efficiency (F) of the reaction. The analytical equation describing the kinetics of PCR that is influenced by template re-annealing is formulated. It predicts the gradual reduction of F - from its initial...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3422235/ https://www.ncbi.nlm.nih.gov/pubmed/22912684 http://dx.doi.org/10.1371/journal.pone.0042063 |