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Accurate Estimation of Nucleic Acids by Amplification Efficiency Dependent PCR

Accurate estimation of template - DNA or RNA by real time PCR is dependent on the amplification efficiency (F) of the reaction. The analytical equation describing the kinetics of PCR that is influenced by template re-annealing is formulated. It predicts the gradual reduction of F - from its initial...

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Detalles Bibliográficos
Autores principales:	Chatterjee, Nilanjana, Banerjee, Tanmay, Datta, Santanu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3422235/ https://www.ncbi.nlm.nih.gov/pubmed/22912684 http://dx.doi.org/10.1371/journal.pone.0042063

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