Avoiding Pitfalls of Internal Controls: Validation of Reference Genes for Analysis by qRT-PCR and Western Blot throughout Rat Retinal Development

BACKGROUND: Housekeeping genes have been commonly used as reference to normalize gene expression and protein content data because of its presumed constitutive expression. In this paper, we challenge the consensual idea that housekeeping genes are reliable controls for expression studies in the retin...

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Detalles Bibliográficos
Autores principales: Rocha-Martins, Maurício, Njaine, Brian, Silveira, Mariana S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3423434/
https://www.ncbi.nlm.nih.gov/pubmed/22916200
http://dx.doi.org/10.1371/journal.pone.0043028

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3423434/
https://www.ncbi.nlm.nih.gov/pubmed/22916200
http://dx.doi.org/10.1371/journal.pone.0043028

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