Cryptic transcripts from a ubiquitous plasmid origin of replication confound tests for cis-regulatory function

A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic promoter within the origin of replication. Readthro...

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Detalles Bibliográficos
Autores principales: Lemp, Nathan A., Hiraoka, Kei, Kasahara, Noriyuki, Logg, Christopher R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Gene Regulation, Chromatin and Epigenetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424574/
https://www.ncbi.nlm.nih.gov/pubmed/22618870
http://dx.doi.org/10.1093/nar/gks451

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424574/
https://www.ncbi.nlm.nih.gov/pubmed/22618870
http://dx.doi.org/10.1093/nar/gks451

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