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Cryptic transcripts from a ubiquitous plasmid origin of replication confound tests for cis-regulatory function
A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic promoter within the origin of replication. Readthro...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424574/ https://www.ncbi.nlm.nih.gov/pubmed/22618870 http://dx.doi.org/10.1093/nar/gks451 |