Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers

DNA built from modular repeats presents a challenge for gene synthesis. We present a solid surface-based sequential ligation approach, which we refer to as iterative capped assembly (ICA), that adds DNA repeat monomers individually to a growing chain while using hairpin ‘capping’ oligonucleotides to...

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Detalles Bibliográficos
Autores principales: Briggs, Adrian W., Rios, Xavier, Chari, Raj, Yang, Luhan, Zhang, Feng, Mali, Prashant, Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424587/
https://www.ncbi.nlm.nih.gov/pubmed/22740649
http://dx.doi.org/10.1093/nar/gks624

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424587/
https://www.ncbi.nlm.nih.gov/pubmed/22740649
http://dx.doi.org/10.1093/nar/gks624

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