Pattern analysis approach reveals restriction enzyme cutting abnormalities and other cDNA library construction artifacts using raw EST data

BACKGROUND: Expressed Sequence Tag (EST) sequences are widely used in applications such as genome annotation, gene discovery and gene expression studies. However, some of GenBank dbEST sequences have proven to be “unclean”. Identification of cDNA termini/ends and their structures in raw ESTs not onl...

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Detalles Bibliográficos
Autores principales: Zhou, Sun, Ji, Guoli, Liu, Xiaolin, Li, Pei, Moler, James, Karro, John E, Liang, Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424822/
https://www.ncbi.nlm.nih.gov/pubmed/22554190
http://dx.doi.org/10.1186/1472-6750-12-16

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424822/
https://www.ncbi.nlm.nih.gov/pubmed/22554190
http://dx.doi.org/10.1186/1472-6750-12-16

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