The Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragment

[Image: see text] Mammalian Nod2 is an intracellular protein that is implicated in the innate immune response to the bacterial cell wall and is associated with the development of Crohn’s disease, Blau syndrome, and gastrointestinal cancers. Nod2 is required for an immune response to muramyl dipeptid...

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Autores principales: Grimes, Catherine Leimkuhler, Ariyananda, Lushanti De Zoysa, Melnyk, James E., O’Shea, Erin K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2012
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424850/
https://www.ncbi.nlm.nih.gov/pubmed/22857257
http://dx.doi.org/10.1021/ja303883c
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author Grimes, Catherine Leimkuhler
Ariyananda, Lushanti De Zoysa
Melnyk, James E.
O’Shea, Erin K.
author_facet Grimes, Catherine Leimkuhler
Ariyananda, Lushanti De Zoysa
Melnyk, James E.
O’Shea, Erin K.
author_sort Grimes, Catherine Leimkuhler
collection PubMed
description [Image: see text] Mammalian Nod2 is an intracellular protein that is implicated in the innate immune response to the bacterial cell wall and is associated with the development of Crohn’s disease, Blau syndrome, and gastrointestinal cancers. Nod2 is required for an immune response to muramyl dipeptide (MDP), an immunostimulatory fragment of bacterial cell wall, but it is not known whether MDP binds directly to Nod2. We report the expression and purification of human Nod2 from insect cells. Using novel MDP self-assembled monolayers (SAMs), we provide the first biochemical evidence for a direct, high-affinity interaction between Nod2 and MDP.
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spelling pubmed-34248502012-08-22 The Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragment Grimes, Catherine Leimkuhler Ariyananda, Lushanti De Zoysa Melnyk, James E. O’Shea, Erin K. J Am Chem Soc [Image: see text] Mammalian Nod2 is an intracellular protein that is implicated in the innate immune response to the bacterial cell wall and is associated with the development of Crohn’s disease, Blau syndrome, and gastrointestinal cancers. Nod2 is required for an immune response to muramyl dipeptide (MDP), an immunostimulatory fragment of bacterial cell wall, but it is not known whether MDP binds directly to Nod2. We report the expression and purification of human Nod2 from insect cells. Using novel MDP self-assembled monolayers (SAMs), we provide the first biochemical evidence for a direct, high-affinity interaction between Nod2 and MDP. American Chemical Society 2012-08-02 2012-08-22 /pmc/articles/PMC3424850/ /pubmed/22857257 http://dx.doi.org/10.1021/ja303883c Text en Copyright © 2012 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.
spellingShingle Grimes, Catherine Leimkuhler
Ariyananda, Lushanti De Zoysa
Melnyk, James E.
O’Shea, Erin K.
The Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragment
title The Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragment
title_full The Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragment
title_fullStr The Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragment
title_full_unstemmed The Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragment
title_short The Innate Immune Protein Nod2 Binds Directly to MDP, a Bacterial Cell Wall Fragment
title_sort innate immune protein nod2 binds directly to mdp, a bacterial cell wall fragment
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424850/
https://www.ncbi.nlm.nih.gov/pubmed/22857257
http://dx.doi.org/10.1021/ja303883c
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