A Concept for Selection of Codon-Suppressor tRNAs Based on Read-Through Ribosome Display in an In Vitro Compartmentalized Cell-Free Translation System

Here is presented a concept for in vitro selection of suppressor tRNAs. It uses a pool of dsDNA templates in compartmentalized water-in-oil micelles. The template contains a transcription/translation trigger, an amber stop codon, and another transcription trigger for the anticodon- or anticodon loop...

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Detalles Bibliográficos
Autores principales: Ogawa, Atsushi, Hayami, Masayoshi, Sando, Shinsuke, Aoyama, Yasuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425794/
https://www.ncbi.nlm.nih.gov/pubmed/22928090
http://dx.doi.org/10.1155/2012/538129

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425794/
https://www.ncbi.nlm.nih.gov/pubmed/22928090
http://dx.doi.org/10.1155/2012/538129

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