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3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus
The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-iso...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428319/ https://www.ncbi.nlm.nih.gov/pubmed/22952592 http://dx.doi.org/10.1371/journal.pone.0042267 |
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author | Lechene, Claude P. Lee, Gloria Y. Poczatek, J. Collin Toner, Mehmet Biggers, John D. |
author_facet | Lechene, Claude P. Lee, Gloria Y. Poczatek, J. Collin Toner, Mehmet Biggers, John D. |
author_sort | Lechene, Claude P. |
collection | PubMed |
description | The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permitting tolerance to desiccation. These results have broad implications for long-term storage of mammalian cells. |
format | Online Article Text |
id | pubmed-3428319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34283192012-09-05 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus Lechene, Claude P. Lee, Gloria Y. Poczatek, J. Collin Toner, Mehmet Biggers, John D. PLoS One Research Article The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permitting tolerance to desiccation. These results have broad implications for long-term storage of mammalian cells. Public Library of Science 2012-08-27 /pmc/articles/PMC3428319/ /pubmed/22952592 http://dx.doi.org/10.1371/journal.pone.0042267 Text en © 2012 Lechene et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lechene, Claude P. Lee, Gloria Y. Poczatek, J. Collin Toner, Mehmet Biggers, John D. 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus |
title | 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus |
title_full | 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus |
title_fullStr | 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus |
title_full_unstemmed | 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus |
title_short | 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus |
title_sort | 3d multi-isotope imaging mass spectrometry reveals penetration of (18)o-trehalose in mouse sperm nucleus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428319/ https://www.ncbi.nlm.nih.gov/pubmed/22952592 http://dx.doi.org/10.1371/journal.pone.0042267 |
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