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3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus

The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-iso...

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Detalles Bibliográficos
Autores principales: Lechene, Claude P., Lee, Gloria Y., Poczatek, J. Collin, Toner, Mehmet, Biggers, John D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428319/
https://www.ncbi.nlm.nih.gov/pubmed/22952592
http://dx.doi.org/10.1371/journal.pone.0042267
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author Lechene, Claude P.
Lee, Gloria Y.
Poczatek, J. Collin
Toner, Mehmet
Biggers, John D.
author_facet Lechene, Claude P.
Lee, Gloria Y.
Poczatek, J. Collin
Toner, Mehmet
Biggers, John D.
author_sort Lechene, Claude P.
collection PubMed
description The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permitting tolerance to desiccation. These results have broad implications for long-term storage of mammalian cells.
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spelling pubmed-34283192012-09-05 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus Lechene, Claude P. Lee, Gloria Y. Poczatek, J. Collin Toner, Mehmet Biggers, John D. PLoS One Research Article The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permitting tolerance to desiccation. These results have broad implications for long-term storage of mammalian cells. Public Library of Science 2012-08-27 /pmc/articles/PMC3428319/ /pubmed/22952592 http://dx.doi.org/10.1371/journal.pone.0042267 Text en © 2012 Lechene et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lechene, Claude P.
Lee, Gloria Y.
Poczatek, J. Collin
Toner, Mehmet
Biggers, John D.
3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus
title 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus
title_full 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus
title_fullStr 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus
title_full_unstemmed 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus
title_short 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of (18)O-Trehalose in Mouse Sperm Nucleus
title_sort 3d multi-isotope imaging mass spectrometry reveals penetration of (18)o-trehalose in mouse sperm nucleus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428319/
https://www.ncbi.nlm.nih.gov/pubmed/22952592
http://dx.doi.org/10.1371/journal.pone.0042267
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