M-Track: detecting short-lived protein-protein interactions in vivo

We developed a protein-proximity assay in yeast based on fusing a histone lysine methyltransferase onto a bait and its substrate onto a prey. Upon binding, the prey is stably methylated and detected by methylation-specific antibodies. We applied this approach to detect varying interaction affinities...

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Detalles Bibliográficos
Autores principales: Zuzuarregui, Aurora, Kupka, Thomas, Bhatt, Bhumika, Dohnal, Ilse, Mudrak, Ingrid, Friedmann, Christina, Schüchner, Stefan, Frohner, Ingrid E., Ammerer, Gustav, Ogris, Egon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428623/
https://www.ncbi.nlm.nih.gov/pubmed/22581371
http://dx.doi.org/10.1038/nmeth.2017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428623/
https://www.ncbi.nlm.nih.gov/pubmed/22581371
http://dx.doi.org/10.1038/nmeth.2017

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