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New cell line development for antibody-producing Chinese hamster ovary cells using split green fluorescent protein
BACKGROUND: The establishment of high producer is an important issue in Chinese hamster ovary (CHO) cell culture considering increased heterogeneity by the random integration of a transfected foreign gene and the altered position of the integrated gene. Fluorescence-activated cell sorting (FACS)-bas...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428690/ https://www.ncbi.nlm.nih.gov/pubmed/22587529 http://dx.doi.org/10.1186/1472-6750-12-24 |