Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission

Optogenetic silencing strategies using light-driven ion fluxes permit rapid and effective inhibition of neural activity. Using rodent hippocampal neurons we show that silencing activity with a chloride pump can increase the probability of synaptically-evoked spiking in the period following light-act...

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Autores principales: Raimondo, Joseph V., Kay, Louise, Ellender, Tommas J., Akerman, Colin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428858/
https://www.ncbi.nlm.nih.gov/pubmed/22729174
http://dx.doi.org/10.1038/nn.3143
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author Raimondo, Joseph V.
Kay, Louise
Ellender, Tommas J.
Akerman, Colin J.
author_facet Raimondo, Joseph V.
Kay, Louise
Ellender, Tommas J.
Akerman, Colin J.
author_sort Raimondo, Joseph V.
collection PubMed
description Optogenetic silencing strategies using light-driven ion fluxes permit rapid and effective inhibition of neural activity. Using rodent hippocampal neurons we show that silencing activity with a chloride pump can increase the probability of synaptically-evoked spiking in the period following light-activation, whereas this is not the case for a proton pump. This effect can be accounted for by changes to the GABA(A) receptor reversal potential and demonstrates an important difference between silencing strategies.
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spelling pubmed-34288582013-02-01 Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission Raimondo, Joseph V. Kay, Louise Ellender, Tommas J. Akerman, Colin J. Nat Neurosci Article Optogenetic silencing strategies using light-driven ion fluxes permit rapid and effective inhibition of neural activity. Using rodent hippocampal neurons we show that silencing activity with a chloride pump can increase the probability of synaptically-evoked spiking in the period following light-activation, whereas this is not the case for a proton pump. This effect can be accounted for by changes to the GABA(A) receptor reversal potential and demonstrates an important difference between silencing strategies. 2012-06-24 /pmc/articles/PMC3428858/ /pubmed/22729174 http://dx.doi.org/10.1038/nn.3143 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Raimondo, Joseph V.
Kay, Louise
Ellender, Tommas J.
Akerman, Colin J.
Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission
title Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission
title_full Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission
title_fullStr Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission
title_full_unstemmed Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission
title_short Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission
title_sort optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428858/
https://www.ncbi.nlm.nih.gov/pubmed/22729174
http://dx.doi.org/10.1038/nn.3143
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