Targeting pre-miRNA by Peptide Nucleic Acids: A new strategy to interfere in the miRNA maturation

PNAs conjugated to carrier peptides have been employed for the targeting of miRNA precursor, with the aim to develop molecules able to interfere in the pre-miRNA processing. The capability of the molecules to bind pre-miRNA has been tested in vitro by fluorescence assayes on Thiazole Orange labeled...

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Autores principales: Avitabile, Concetta, Saviano, Michele, D'Andrea, Luca, Bianchi, Nicoletta, Fabbri, Enrica, Brognara, Eleonora, Gambari, Roberto, Romanelli, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2012
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429535/
https://www.ncbi.nlm.nih.gov/pubmed/22699795
http://dx.doi.org/10.4161/adna.20911
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author Avitabile, Concetta
Saviano, Michele
D'Andrea, Luca
Bianchi, Nicoletta
Fabbri, Enrica
Brognara, Eleonora
Gambari, Roberto
Romanelli, Alessandra
author_facet Avitabile, Concetta
Saviano, Michele
D'Andrea, Luca
Bianchi, Nicoletta
Fabbri, Enrica
Brognara, Eleonora
Gambari, Roberto
Romanelli, Alessandra
author_sort Avitabile, Concetta
collection PubMed
description PNAs conjugated to carrier peptides have been employed for the targeting of miRNA precursor, with the aim to develop molecules able to interfere in the pre-miRNA processing. The capability of the molecules to bind pre-miRNA has been tested in vitro by fluorescence assayes on Thiazole Orange labeled molecules and in vivo, in K562 cells, evaluating the amount of miRNA produced after treatment of cells with two amounts of PNAs.
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spelling pubmed-34295352012-08-29 Targeting pre-miRNA by Peptide Nucleic Acids: A new strategy to interfere in the miRNA maturation Avitabile, Concetta Saviano, Michele D'Andrea, Luca Bianchi, Nicoletta Fabbri, Enrica Brognara, Eleonora Gambari, Roberto Romanelli, Alessandra Artif DNA PNA XNA Research Paper PNAs conjugated to carrier peptides have been employed for the targeting of miRNA precursor, with the aim to develop molecules able to interfere in the pre-miRNA processing. The capability of the molecules to bind pre-miRNA has been tested in vitro by fluorescence assayes on Thiazole Orange labeled molecules and in vivo, in K562 cells, evaluating the amount of miRNA produced after treatment of cells with two amounts of PNAs. Landes Bioscience 2012-04-01 /pmc/articles/PMC3429535/ /pubmed/22699795 http://dx.doi.org/10.4161/adna.20911 Text en Copyright © 2012 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Research Paper
Avitabile, Concetta
Saviano, Michele
D'Andrea, Luca
Bianchi, Nicoletta
Fabbri, Enrica
Brognara, Eleonora
Gambari, Roberto
Romanelli, Alessandra
Targeting pre-miRNA by Peptide Nucleic Acids: A new strategy to interfere in the miRNA maturation
title Targeting pre-miRNA by Peptide Nucleic Acids: A new strategy to interfere in the miRNA maturation
title_full Targeting pre-miRNA by Peptide Nucleic Acids: A new strategy to interfere in the miRNA maturation
title_fullStr Targeting pre-miRNA by Peptide Nucleic Acids: A new strategy to interfere in the miRNA maturation
title_full_unstemmed Targeting pre-miRNA by Peptide Nucleic Acids: A new strategy to interfere in the miRNA maturation
title_short Targeting pre-miRNA by Peptide Nucleic Acids: A new strategy to interfere in the miRNA maturation
title_sort targeting pre-mirna by peptide nucleic acids: a new strategy to interfere in the mirna maturation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429535/
https://www.ncbi.nlm.nih.gov/pubmed/22699795
http://dx.doi.org/10.4161/adna.20911
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