Random and cyclical deletion of large DNA segments in the genome of Pseudomonas putida

Cumulative site-directed mutagenesis is of limited suitability for the global analysis of the gene functions in the microbe's cellular network. In order to simplify and stabilize the genome of the soil bacterium Pseudomonas putida, we developed a recyclable three-step excision method based on t...

Descripción completa

Detalles Bibliográficos
Autores principales: Leprince, Audrey, de Lorenzo, Víctor, Völler, Petra, van Passel, Mark W J, Martins dos Santos, Vitor A P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429869/
https://www.ncbi.nlm.nih.gov/pubmed/22429517
http://dx.doi.org/10.1111/j.1462-2920.2012.02730.x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429869/
https://www.ncbi.nlm.nih.gov/pubmed/22429517
http://dx.doi.org/10.1111/j.1462-2920.2012.02730.x

Ejemplares similares