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Random and cyclical deletion of large DNA segments in the genome of Pseudomonas putida
Cumulative site-directed mutagenesis is of limited suitability for the global analysis of the gene functions in the microbe's cellular network. In order to simplify and stabilize the genome of the soil bacterium Pseudomonas putida, we developed a recyclable three-step excision method based on t...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Blackwell Publishing Ltd
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429869/ https://www.ncbi.nlm.nih.gov/pubmed/22429517 http://dx.doi.org/10.1111/j.1462-2920.2012.02730.x |