Tryptophan-based chromophore in fluorescent proteins can be anionic

Cyan fluorescent proteins (CFP) with tryptophan66-based chromophore are widely used for live cell imaging. In contrast to green and red fluorescent proteins, no charged states of the CFP chromophore have been described. Here, we studied synthetic CFP chromophore and found that its indole group can b...

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Detalles Bibliográficos
Autores principales: Sarkisyan, Karen S., Yampolsky, Ilia V., Solntsev, Kyril M., Lukyanov, Sergey A., Lukyanov, Konstantin A., Mishin, Alexander S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429880/
https://www.ncbi.nlm.nih.gov/pubmed/22934131
http://dx.doi.org/10.1038/srep00608

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429880/
https://www.ncbi.nlm.nih.gov/pubmed/22934131
http://dx.doi.org/10.1038/srep00608

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