A Protease for Middle Down Proteomics

We developed a method for restricted enzymatic proteolysis using the outer membrane protease T (OmpT) to produce large peptides (> 6.3 kDa on average) for mass spectrometry-based proteomics. Using this approach to analyze prefractionated high-mass HeLa proteins we identified 3,697 unique peptides...

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Detalles Bibliográficos
Autores principales: Wu, Cong, Tran, John C., Zamdborg, Leonid, Durbin, Kenneth R., Li, Mingxi, Ahlf, Dorothy R., Early, Bryan P., Thomas, Paul M., Sweedler, Jonathan V., Kelleher, Neil L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430368/
https://www.ncbi.nlm.nih.gov/pubmed/22706673
http://dx.doi.org/10.1038/nmeth.2074

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430368/
https://www.ncbi.nlm.nih.gov/pubmed/22706673
http://dx.doi.org/10.1038/nmeth.2074

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