A scalable pipeline for highly effective genetic modification of a malaria parasite

In malaria parasites the systematic experimental validation of drug and vaccine targets by reverse genetics is constrained by the inefficiency of homologous recombination and by the difficulty of manipulating adenine and thymine (AT) rich Plasmodium DNA in E. coli. We overcome these roadblocks by de...

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Detalles Bibliográficos
Autores principales: Pfander, Claudia, Anar, Burcu, Schwach, Frank, Otto, Thomas D., Brochet, Mathieu, Volkmann, Katrin, Quail, Michael A., Pain, Arnab, Rosen, Barry, Skarnes, William, Rayner, Julian C., Billker, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431185/
https://www.ncbi.nlm.nih.gov/pubmed/22020067
http://dx.doi.org/10.1038/nmeth.1742

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431185/
https://www.ncbi.nlm.nih.gov/pubmed/22020067
http://dx.doi.org/10.1038/nmeth.1742

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