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Development of Microsatellite Markers Using Pyrosequencing in Galium trifidum (Rubiaceae), a Rare Species in Central Europe

We identify a large number of microsatellites from Galium trfidum, a plant species considered rare and endangered in Central and Western Europe. Using a combination of a total enriched genomic library and small-scale 454 pyrosequencing, we determined 9755 contigs with a length of 100 to 6192 bp. Wit...

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Detalles Bibliográficos
Autores principales: Szczecińska, Monika, Kwaśniewski, Mirosław, Sawicki, Jakub, Chwiałkowska, Karolina, Szandar, Kamil, Pisarek, Włodzimierz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431835/
https://www.ncbi.nlm.nih.gov/pubmed/22949837
http://dx.doi.org/10.3390/ijms13089893