Direct observation of mammalian cell growth and size regulation

We introduce a microfluidic system for simultaneously measuring single cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 hours of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in...

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Detalles Bibliográficos
Autores principales: Son, Sungmin, Tzur, Amit, Weng, Yaochung, Jorgensen, Paul, Kim, Jisoo, Kirschner, Marc W., Manalis, Scott R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433595/
https://www.ncbi.nlm.nih.gov/pubmed/22863882
http://dx.doi.org/10.1038/nmeth.2133
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author Son, Sungmin
Tzur, Amit
Weng, Yaochung
Jorgensen, Paul
Kim, Jisoo
Kirschner, Marc W.
Manalis, Scott R.
author_facet Son, Sungmin
Tzur, Amit
Weng, Yaochung
Jorgensen, Paul
Kim, Jisoo
Kirschner, Marc W.
Manalis, Scott R.
author_sort Son, Sungmin
collection PubMed
description We introduce a microfluidic system for simultaneously measuring single cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 hours of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1/S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.
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spelling pubmed-34335952013-03-01 Direct observation of mammalian cell growth and size regulation Son, Sungmin Tzur, Amit Weng, Yaochung Jorgensen, Paul Kim, Jisoo Kirschner, Marc W. Manalis, Scott R. Nat Methods Article We introduce a microfluidic system for simultaneously measuring single cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 hours of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1/S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis. 2012-08-05 2012-09 /pmc/articles/PMC3433595/ /pubmed/22863882 http://dx.doi.org/10.1038/nmeth.2133 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Son, Sungmin
Tzur, Amit
Weng, Yaochung
Jorgensen, Paul
Kim, Jisoo
Kirschner, Marc W.
Manalis, Scott R.
Direct observation of mammalian cell growth and size regulation
title Direct observation of mammalian cell growth and size regulation
title_full Direct observation of mammalian cell growth and size regulation
title_fullStr Direct observation of mammalian cell growth and size regulation
title_full_unstemmed Direct observation of mammalian cell growth and size regulation
title_short Direct observation of mammalian cell growth and size regulation
title_sort direct observation of mammalian cell growth and size regulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433595/
https://www.ncbi.nlm.nih.gov/pubmed/22863882
http://dx.doi.org/10.1038/nmeth.2133
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