Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules

We present dial-out PCR, a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequence...

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Detalles Bibliográficos
Autores principales: Schwartz, Jerrod J, Lee, Choli, Shendure, Jay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433648/
https://www.ncbi.nlm.nih.gov/pubmed/22886093
http://dx.doi.org/10.1038/nmeth.2137

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433648/
https://www.ncbi.nlm.nih.gov/pubmed/22886093
http://dx.doi.org/10.1038/nmeth.2137

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