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Uncoupling the roles of synaptotagmin I as a dual Ca(2+) sensor during endo- and exocytosis of synaptic vesicles
Synaptotagmin I (syt1) is required for normal rates of synaptic vesicle endo- and exocytosis. However, whether the kinetic defects observed during endocytosis in syt1 knock-out neurons are secondary to defective exocytosis, or whether syt1 directly regulates the rate of vesicle retrieval, remains un...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3435110/ https://www.ncbi.nlm.nih.gov/pubmed/22197832 http://dx.doi.org/10.1038/nn.3013 |
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author | Yao, Jun Kwon, Sung E. Gaffaney, Jon D. Dunning, F. Mark Chapman, Edwin R. |
author_facet | Yao, Jun Kwon, Sung E. Gaffaney, Jon D. Dunning, F. Mark Chapman, Edwin R. |
author_sort | Yao, Jun |
collection | PubMed |
description | Synaptotagmin I (syt1) is required for normal rates of synaptic vesicle endo- and exocytosis. However, whether the kinetic defects observed during endocytosis in syt1 knock-out neurons are secondary to defective exocytosis, or whether syt1 directly regulates the rate of vesicle retrieval, remains unresolved. In order to address this question, it is necessary to dissociate these two activities. Here, we have uncoupled the function of syt1 in exo- and endocytosis by re-targeting of the protein, or via mutagenesis of its tandem C2-domains; the impact of these manipulations on exo- and endocytosis were analyzed via electrophysiology, in conjunction with optical imaging of the vesicle cycle. These experiments uncovered a direct role for syt1 in endocytosis. Surprisingly, either C2-domain of syt1 - C2A or C2B - was able to function as Ca(2+)-sensor for endocytosis. Hence, syt1 functions as a dual Ca(2+) sensor for both endo- and exocytosis, potentially coupling these two limbs of the vesicle cycle. |
format | Online Article Text |
id | pubmed-3435110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
record_format | MEDLINE/PubMed |
spelling | pubmed-34351102012-09-06 Uncoupling the roles of synaptotagmin I as a dual Ca(2+) sensor during endo- and exocytosis of synaptic vesicles Yao, Jun Kwon, Sung E. Gaffaney, Jon D. Dunning, F. Mark Chapman, Edwin R. Nat Neurosci Article Synaptotagmin I (syt1) is required for normal rates of synaptic vesicle endo- and exocytosis. However, whether the kinetic defects observed during endocytosis in syt1 knock-out neurons are secondary to defective exocytosis, or whether syt1 directly regulates the rate of vesicle retrieval, remains unresolved. In order to address this question, it is necessary to dissociate these two activities. Here, we have uncoupled the function of syt1 in exo- and endocytosis by re-targeting of the protein, or via mutagenesis of its tandem C2-domains; the impact of these manipulations on exo- and endocytosis were analyzed via electrophysiology, in conjunction with optical imaging of the vesicle cycle. These experiments uncovered a direct role for syt1 in endocytosis. Surprisingly, either C2-domain of syt1 - C2A or C2B - was able to function as Ca(2+)-sensor for endocytosis. Hence, syt1 functions as a dual Ca(2+) sensor for both endo- and exocytosis, potentially coupling these two limbs of the vesicle cycle. 2011-12-25 /pmc/articles/PMC3435110/ /pubmed/22197832 http://dx.doi.org/10.1038/nn.3013 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Yao, Jun Kwon, Sung E. Gaffaney, Jon D. Dunning, F. Mark Chapman, Edwin R. Uncoupling the roles of synaptotagmin I as a dual Ca(2+) sensor during endo- and exocytosis of synaptic vesicles |
title | Uncoupling the roles of synaptotagmin I as a dual Ca(2+) sensor during endo- and exocytosis of synaptic vesicles |
title_full | Uncoupling the roles of synaptotagmin I as a dual Ca(2+) sensor during endo- and exocytosis of synaptic vesicles |
title_fullStr | Uncoupling the roles of synaptotagmin I as a dual Ca(2+) sensor during endo- and exocytosis of synaptic vesicles |
title_full_unstemmed | Uncoupling the roles of synaptotagmin I as a dual Ca(2+) sensor during endo- and exocytosis of synaptic vesicles |
title_short | Uncoupling the roles of synaptotagmin I as a dual Ca(2+) sensor during endo- and exocytosis of synaptic vesicles |
title_sort | uncoupling the roles of synaptotagmin i as a dual ca(2+) sensor during endo- and exocytosis of synaptic vesicles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3435110/ https://www.ncbi.nlm.nih.gov/pubmed/22197832 http://dx.doi.org/10.1038/nn.3013 |
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