Groundtruthing Next-Gen Sequencing for Microbial Ecology–Biases and Errors in Community Structure Estimates from PCR Amplicon Pyrosequencing

Analysis of microbial communities by high-throughput pyrosequencing of SSU rRNA gene PCR amplicons has transformed microbial ecology research and led to the observation that many communities contain a diverse assortment of rare taxa–a phenomenon termed the Rare Biosphere. Multiple studies have inves...

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Detalles Bibliográficos
Autores principales: Lee, Charles K., Herbold, Craig W., Polson, Shawn W., Wommack, K. Eric, Williamson, Shannon J., McDonald, Ian R., Cary, S. Craig
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3435322/
https://www.ncbi.nlm.nih.gov/pubmed/22970184
http://dx.doi.org/10.1371/journal.pone.0044224

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3435322/
https://www.ncbi.nlm.nih.gov/pubmed/22970184
http://dx.doi.org/10.1371/journal.pone.0044224

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