Direct visualization of specifically modified extracellular glycans in living animals

Modification patterns of the extracellular glycan heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific hep...

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Autores principales: Attreed, Matthew, Desbois, Muriel, van Kuppevelt, Toin H., Bülow, Hannes E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437987/
https://www.ncbi.nlm.nih.gov/pubmed/22466794
http://dx.doi.org/10.1038/nmeth.1945
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author Attreed, Matthew
Desbois, Muriel
van Kuppevelt, Toin H.
Bülow, Hannes E.
author_facet Attreed, Matthew
Desbois, Muriel
van Kuppevelt, Toin H.
Bülow, Hannes E.
author_sort Attreed, Matthew
collection PubMed
description Modification patterns of the extracellular glycan heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.
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spelling pubmed-34379872012-11-01 Direct visualization of specifically modified extracellular glycans in living animals Attreed, Matthew Desbois, Muriel van Kuppevelt, Toin H. Bülow, Hannes E. Nat Methods Article Modification patterns of the extracellular glycan heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo. 2012-04-01 /pmc/articles/PMC3437987/ /pubmed/22466794 http://dx.doi.org/10.1038/nmeth.1945 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Attreed, Matthew
Desbois, Muriel
van Kuppevelt, Toin H.
Bülow, Hannes E.
Direct visualization of specifically modified extracellular glycans in living animals
title Direct visualization of specifically modified extracellular glycans in living animals
title_full Direct visualization of specifically modified extracellular glycans in living animals
title_fullStr Direct visualization of specifically modified extracellular glycans in living animals
title_full_unstemmed Direct visualization of specifically modified extracellular glycans in living animals
title_short Direct visualization of specifically modified extracellular glycans in living animals
title_sort direct visualization of specifically modified extracellular glycans in living animals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437987/
https://www.ncbi.nlm.nih.gov/pubmed/22466794
http://dx.doi.org/10.1038/nmeth.1945
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