Random Mutagenesis MAPPIT Analysis Identifies Binding Sites for Vif and Gag in Both Cytidine Deaminase Domains of Apobec3G

The mammalian two-hybrid system MAPPIT allows the detection of protein-protein interactions in intact human cells. We developed a random mutagenesis screening strategy based on MAPPIT to detect mutations that disrupt the interaction of one protein with multiple protein interactors simultanously. The...

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Detalles Bibliográficos
Autores principales: Uyttendaele, Isabel, Lavens, Delphine, Catteeuw, Dominiek, Lemmens, Irma, Bovijn, Celia, Tavernier, Jan, Peelman, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3438196/
https://www.ncbi.nlm.nih.gov/pubmed/22970171
http://dx.doi.org/10.1371/journal.pone.0044143

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3438196/
https://www.ncbi.nlm.nih.gov/pubmed/22970171
http://dx.doi.org/10.1371/journal.pone.0044143

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