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Flavin Mononucleotide-Based Fluorescent Proteins Function in Mammalian Cells without Oxygen Requirement

Usage of the enhanced green fluorescent protein (eGFP) in living mammalian cells is limited to aerobic conditions due to requirement of oxygen during chromophore formation. Since many diseases or disease models are associated with acute or chronic hypoxia, eGFP-labeling of structures of interest in...

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Detalles Bibliográficos
Autores principales: Walter, Janine, Hausmann, Sascha, Drepper, Thomas, Puls, Michael, Eggert, Thorsten, Dihné, Marcel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439463/
https://www.ncbi.nlm.nih.gov/pubmed/22984451
http://dx.doi.org/10.1371/journal.pone.0043921