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Flavin Mononucleotide-Based Fluorescent Proteins Function in Mammalian Cells without Oxygen Requirement
Usage of the enhanced green fluorescent protein (eGFP) in living mammalian cells is limited to aerobic conditions due to requirement of oxygen during chromophore formation. Since many diseases or disease models are associated with acute or chronic hypoxia, eGFP-labeling of structures of interest in...
Autores principales: | Walter, Janine, Hausmann, Sascha, Drepper, Thomas, Puls, Michael, Eggert, Thorsten, Dihné, Marcel |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439463/ https://www.ncbi.nlm.nih.gov/pubmed/22984451 http://dx.doi.org/10.1371/journal.pone.0043921 |
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