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An Economical Method for Production of (2)H,(13)CH(3)-Threonine for Solution NMR Studies of Large Protein Complexes: Application to the 670 kDa Proteasome
NMR studies of very high molecular weight protein complexes have been greatly facilitated through the development of labeling strategies whereby (13)CH(3) methyl groups are introduced into highly deuterated proteins. Robust and cost-effective labeling methods are well established for all methyl cont...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3439479/ https://www.ncbi.nlm.nih.gov/pubmed/22984438 http://dx.doi.org/10.1371/journal.pone.0043725 |