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Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry

BACKGROUND: The fabrication of recombinant collagen and its prescribed variants has enormous potential in tissue regeneration, cell-matrix interaction investigations, and fundamental biochemical and biophysical studies of the extracellular matrix. Recombinant expression requires proline hydroxylatio...

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Detalles Bibliográficos
Autores principales: Chan, S W Polly, Greaves, John, Da Silva, Nancy A, Wang, Szu-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3443662/
https://www.ncbi.nlm.nih.gov/pubmed/22901055
http://dx.doi.org/10.1186/1472-6750-12-51