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Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation

The cytoplasmic dynein motor generates pulling forces to center and orient the mitotic spindle within the cell. During this positioning process, dynein oscillates from one pole of the cell cortex to the other but only accumulates at the pole farthest from the spindle. Here, we show that dynein light...

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Autores principales: Dunsch, Anja K., Hammond, Dean, Lloyd, Jennifer, Schermelleh, Lothar, Gruneberg, Ulrike, Barr, Francis A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444778/
https://www.ncbi.nlm.nih.gov/pubmed/22965910
http://dx.doi.org/10.1083/jcb.201202112
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author Dunsch, Anja K.
Hammond, Dean
Lloyd, Jennifer
Schermelleh, Lothar
Gruneberg, Ulrike
Barr, Francis A.
author_facet Dunsch, Anja K.
Hammond, Dean
Lloyd, Jennifer
Schermelleh, Lothar
Gruneberg, Ulrike
Barr, Francis A.
author_sort Dunsch, Anja K.
collection PubMed
description The cytoplasmic dynein motor generates pulling forces to center and orient the mitotic spindle within the cell. During this positioning process, dynein oscillates from one pole of the cell cortex to the other but only accumulates at the pole farthest from the spindle. Here, we show that dynein light chain 1 (DYNLL1) is required for this asymmetric cortical localization of dynein and has a specific function defining spindle orientation. DYNLL1 interacted with a spindle-microtubule–associated adaptor formed by CHICA and HMMR via TQT motifs in CHICA. In cells depleted of CHICA or HMMR, the mitotic spindle failed to orient correctly in relation to the growth surface. Furthermore, CHICA TQT motif mutants localized to the mitotic spindle but failed to recruit DYNLL1 to spindle microtubules and did not correct the spindle orientation or dynein localization defects. These findings support a model where DYNLL1 and CHICA-HMMR form part of the regulatory system feeding back spindle position to dynein at the cell cortex.
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spelling pubmed-34447782013-03-17 Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation Dunsch, Anja K. Hammond, Dean Lloyd, Jennifer Schermelleh, Lothar Gruneberg, Ulrike Barr, Francis A. J Cell Biol Research Articles The cytoplasmic dynein motor generates pulling forces to center and orient the mitotic spindle within the cell. During this positioning process, dynein oscillates from one pole of the cell cortex to the other but only accumulates at the pole farthest from the spindle. Here, we show that dynein light chain 1 (DYNLL1) is required for this asymmetric cortical localization of dynein and has a specific function defining spindle orientation. DYNLL1 interacted with a spindle-microtubule–associated adaptor formed by CHICA and HMMR via TQT motifs in CHICA. In cells depleted of CHICA or HMMR, the mitotic spindle failed to orient correctly in relation to the growth surface. Furthermore, CHICA TQT motif mutants localized to the mitotic spindle but failed to recruit DYNLL1 to spindle microtubules and did not correct the spindle orientation or dynein localization defects. These findings support a model where DYNLL1 and CHICA-HMMR form part of the regulatory system feeding back spindle position to dynein at the cell cortex. The Rockefeller University Press 2012-09-17 /pmc/articles/PMC3444778/ /pubmed/22965910 http://dx.doi.org/10.1083/jcb.201202112 Text en © 2012 Dunsch et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Dunsch, Anja K.
Hammond, Dean
Lloyd, Jennifer
Schermelleh, Lothar
Gruneberg, Ulrike
Barr, Francis A.
Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation
title Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation
title_full Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation
title_fullStr Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation
title_full_unstemmed Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation
title_short Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation
title_sort dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444778/
https://www.ncbi.nlm.nih.gov/pubmed/22965910
http://dx.doi.org/10.1083/jcb.201202112
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