Quantitative Fluorescent Labeling of Aldehyde-Tagged Proteins for Single-Molecule Imaging

A major hurdle for molecular mechanistic studies of many proteins is the lack of a general method for fluorescent labeling with high efficiency, specificity, and speed. By incorporating an aldehyde motif genetically into a protein and improving the labeling kinetics substantially under mild conditio...

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Detalles Bibliográficos
Autores principales: Shi, Xinghua, Jung, Yonil, Lin, Li-Jung, Liu, Cheng, Wu, Cong, Cann, Isaac K. O., Ha, Taekjip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445270/
https://www.ncbi.nlm.nih.gov/pubmed/22466795
http://dx.doi.org/10.1038/nmeth.1954

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445270/
https://www.ncbi.nlm.nih.gov/pubmed/22466795
http://dx.doi.org/10.1038/nmeth.1954

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