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Quantitative Fluorescent Labeling of Aldehyde-Tagged Proteins for Single-Molecule Imaging
A major hurdle for molecular mechanistic studies of many proteins is the lack of a general method for fluorescent labeling with high efficiency, specificity, and speed. By incorporating an aldehyde motif genetically into a protein and improving the labeling kinetics substantially under mild conditio...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445270/ https://www.ncbi.nlm.nih.gov/pubmed/22466795 http://dx.doi.org/10.1038/nmeth.1954 |