Analysis of conditions affecting auto-phosphorylation of human kinases during expression in bacteria

Bacterial over-expression of kinases is often associated with high levels of auto-phosphorylation resulting in heterogeneous recombinant protein preparations or sometimes in insoluble protein. Here we present expression systems for nine kinases in Escherichia coli and, for the most heavily phosphory...

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Detalles Bibliográficos
Autores principales: Shrestha, Amit, Hamilton, Garth, O’Neill, Eric, Knapp, Stefan, Elkins, Jonathan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445812/
https://www.ncbi.nlm.nih.gov/pubmed/21985771
http://dx.doi.org/10.1016/j.pep.2011.09.012

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445812/
https://www.ncbi.nlm.nih.gov/pubmed/21985771
http://dx.doi.org/10.1016/j.pep.2011.09.012

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