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Analysis of conditions affecting auto-phosphorylation of human kinases during expression in bacteria
Bacterial over-expression of kinases is often associated with high levels of auto-phosphorylation resulting in heterogeneous recombinant protein preparations or sometimes in insoluble protein. Here we present expression systems for nine kinases in Escherichia coli and, for the most heavily phosphory...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Academic Press
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445812/ https://www.ncbi.nlm.nih.gov/pubmed/21985771 http://dx.doi.org/10.1016/j.pep.2011.09.012 |