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Development of TaqMan Probe-Based Insulated Isothermal PCR (iiPCR) for Sensitive and Specific On-Site Pathogen Detection

Insulated isothermal PCR (iiPCR), established on the basis of Ralyeigh-Bénard convection, is a rapid and low-cost platform for nucleic acid amplification. However, the method used for signal detection, namely gel electrophoresis, has limited the application of iiPCR. In this study, TaqMan probe-base...

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Detalles Bibliográficos
Autores principales: Tsai, Yun-Long, Wang, Hwa-Tang Thomas, Chang, Hsiao-Fen Grace, Tsai, Chuan-Fu, Lin, Ching-Ko, Teng, Ping-Hua, Su, Chen, Jeng, Chien-Chung, Lee, Pei-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3458002/
https://www.ncbi.nlm.nih.gov/pubmed/23049781
http://dx.doi.org/10.1371/journal.pone.0045278