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Dynamics of transcription driven by the tetA promoter, one event at a time, in live Escherichia coli cells
In Escherichia coli, tetracycline prevents translation. When subject to tetracycline, E. coli express TetA to pump it out by a mechanism that is sensitive, while fairly independent of cellular metabolism. We constructed a target gene, P(tetA)-mRFP1-96BS, with a 96 MS2-GFP binding site array in a sin...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3458540/ https://www.ncbi.nlm.nih.gov/pubmed/22730294 http://dx.doi.org/10.1093/nar/gks583 |