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Combined computational and experimental analysis reveals mitogen-activated protein kinase–mediated feedback phosphorylation as a mechanism for signaling specificity

Different environmental stimuli often use the same set of signaling proteins to achieve very different physiological outcomes. The mating and invasive growth pathways in yeast each employ a mitogen-activated protein (MAP) kinase cascade that includes Ste20, Ste11, and Ste7. Whereas proper mating req...

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Autores principales: Hao, Nan, Yildirim, Necmettin, Nagiec, Michal J., Parnell, Stephen C., Errede, Beverly, Dohlman, Henrik G., Elston, Timothy C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459865/
https://www.ncbi.nlm.nih.gov/pubmed/22875986
http://dx.doi.org/10.1091/mbc.E12-04-0333
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author Hao, Nan
Yildirim, Necmettin
Nagiec, Michal J.
Parnell, Stephen C.
Errede, Beverly
Dohlman, Henrik G.
Elston, Timothy C.
author_facet Hao, Nan
Yildirim, Necmettin
Nagiec, Michal J.
Parnell, Stephen C.
Errede, Beverly
Dohlman, Henrik G.
Elston, Timothy C.
author_sort Hao, Nan
collection PubMed
description Different environmental stimuli often use the same set of signaling proteins to achieve very different physiological outcomes. The mating and invasive growth pathways in yeast each employ a mitogen-activated protein (MAP) kinase cascade that includes Ste20, Ste11, and Ste7. Whereas proper mating requires Ste7 activation of the MAP kinase Fus3, invasive growth requires activation of the alternate MAP kinase Kss1. To determine how MAP kinase specificity is achieved, we used a series of mathematical models to quantitatively characterize pheromone-stimulated kinase activation. In accordance with the computational analysis, MAP kinase feedback phosphorylation of Ste7 results in diminished activation of Kss1, but not Fus3. These findings reveal how feedback phosphorylation of a common pathway component can limit the activity of a competing MAP kinase through feedback phosphorylation of a common activator, and thereby promote signal fidelity.
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spelling pubmed-34598652012-12-16 Combined computational and experimental analysis reveals mitogen-activated protein kinase–mediated feedback phosphorylation as a mechanism for signaling specificity Hao, Nan Yildirim, Necmettin Nagiec, Michal J. Parnell, Stephen C. Errede, Beverly Dohlman, Henrik G. Elston, Timothy C. Mol Biol Cell Articles Different environmental stimuli often use the same set of signaling proteins to achieve very different physiological outcomes. The mating and invasive growth pathways in yeast each employ a mitogen-activated protein (MAP) kinase cascade that includes Ste20, Ste11, and Ste7. Whereas proper mating requires Ste7 activation of the MAP kinase Fus3, invasive growth requires activation of the alternate MAP kinase Kss1. To determine how MAP kinase specificity is achieved, we used a series of mathematical models to quantitatively characterize pheromone-stimulated kinase activation. In accordance with the computational analysis, MAP kinase feedback phosphorylation of Ste7 results in diminished activation of Kss1, but not Fus3. These findings reveal how feedback phosphorylation of a common pathway component can limit the activity of a competing MAP kinase through feedback phosphorylation of a common activator, and thereby promote signal fidelity. The American Society for Cell Biology 2012-10-01 /pmc/articles/PMC3459865/ /pubmed/22875986 http://dx.doi.org/10.1091/mbc.E12-04-0333 Text en © 2012 Hao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Hao, Nan
Yildirim, Necmettin
Nagiec, Michal J.
Parnell, Stephen C.
Errede, Beverly
Dohlman, Henrik G.
Elston, Timothy C.
Combined computational and experimental analysis reveals mitogen-activated protein kinase–mediated feedback phosphorylation as a mechanism for signaling specificity
title Combined computational and experimental analysis reveals mitogen-activated protein kinase–mediated feedback phosphorylation as a mechanism for signaling specificity
title_full Combined computational and experimental analysis reveals mitogen-activated protein kinase–mediated feedback phosphorylation as a mechanism for signaling specificity
title_fullStr Combined computational and experimental analysis reveals mitogen-activated protein kinase–mediated feedback phosphorylation as a mechanism for signaling specificity
title_full_unstemmed Combined computational and experimental analysis reveals mitogen-activated protein kinase–mediated feedback phosphorylation as a mechanism for signaling specificity
title_short Combined computational and experimental analysis reveals mitogen-activated protein kinase–mediated feedback phosphorylation as a mechanism for signaling specificity
title_sort combined computational and experimental analysis reveals mitogen-activated protein kinase–mediated feedback phosphorylation as a mechanism for signaling specificity
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459865/
https://www.ncbi.nlm.nih.gov/pubmed/22875986
http://dx.doi.org/10.1091/mbc.E12-04-0333
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