Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates

Proteins of the Rho family of small GTPases are central regulators of the cytoskeleton, and control a large variety of cellular processes, including cell migration, gene expression, cell cycle progression and cell adhesion (1). Rho proteins are molecular switches that are active in GTP-bound and ina...

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Detalles Bibliográficos
Autores principales: Waheed, Faiza, Speight, Pamela, Dan, Qinghong, Garcia-Mata, Rafael, Szaszi, Katalin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2012
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460580/
https://www.ncbi.nlm.nih.gov/pubmed/22491204
http://dx.doi.org/10.3791/3932

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460580/
https://www.ncbi.nlm.nih.gov/pubmed/22491204
http://dx.doi.org/10.3791/3932

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