Resolution Doubling in Live, Multicellular Organisms via Multifocal Structured Illumination Microscopy

We demonstrate 3D super-resolution in live multicellular organisms using structured illumination microscopy (SIM). Sparse multifocal illumination patterns generated by a digital micromirror device (DMD) let us physically reject out-of-focus light, enabling 3D subdiffractive imaging in samples 8-fold...

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Detalles Bibliográficos
Autores principales: York, Andrew G., Parekh, Sapun H., Nogare, Damian Dalle, Fischer, Robert S., Temprine, Kelsey, Mione, Marina, Chitnis, Ajay B., Combs, Christian A., Shroff, Hari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462167/
https://www.ncbi.nlm.nih.gov/pubmed/22581372
http://dx.doi.org/10.1038/nmeth.2025

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462167/
https://www.ncbi.nlm.nih.gov/pubmed/22581372
http://dx.doi.org/10.1038/nmeth.2025

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