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Resolution Doubling in Live, Multicellular Organisms via Multifocal Structured Illumination Microscopy

We demonstrate 3D super-resolution in live multicellular organisms using structured illumination microscopy (SIM). Sparse multifocal illumination patterns generated by a digital micromirror device (DMD) let us physically reject out-of-focus light, enabling 3D subdiffractive imaging in samples 8-fold...

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Detalles Bibliográficos
Autores principales:	York, Andrew G., Parekh, Sapun H., Nogare, Damian Dalle, Fischer, Robert S., Temprine, Kelsey, Mione, Marina, Chitnis, Ajay B., Combs, Christian A., Shroff, Hari
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2012
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462167/ https://www.ncbi.nlm.nih.gov/pubmed/22581372 http://dx.doi.org/10.1038/nmeth.2025

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