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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Due to the high costs associated with purification of recombinant proteins the protocols need to be rationalized. For high-throughput efforts there is a demand for general methods that do not require target protein specific optimization(1) . To achieve this, purification tags that genetically can be...

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Detalles Bibliográficos
Autores principales: Nilvebrant, Johan, Alm, Tove, Hober, Sophia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462565/
https://www.ncbi.nlm.nih.gov/pubmed/22297419
http://dx.doi.org/10.3791/3370