In-house preparation of hydrogels for batch affinity purification of glutathione S-transferase tagged recombinant proteins

BACKGROUND: Many branches of biomedical research find use for pure recombinant proteins for direct application or to study other molecules and pathways. Glutathione affinity purification is commonly used to isolate and purify glutathione S-transferase (GST)-tagged fusion proteins from total cellular...

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Detalles Bibliográficos
Autores principales: Buhrman, Jason S, Rayahin, Jamie E, Köllmer, Melanie, Gemeinhart, Richard A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463477/
https://www.ncbi.nlm.nih.gov/pubmed/22989306
http://dx.doi.org/10.1186/1472-6750-12-63

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463477/
https://www.ncbi.nlm.nih.gov/pubmed/22989306
http://dx.doi.org/10.1186/1472-6750-12-63

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