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In-house preparation of hydrogels for batch affinity purification of glutathione S-transferase tagged recombinant proteins
BACKGROUND: Many branches of biomedical research find use for pure recombinant proteins for direct application or to study other molecules and pathways. Glutathione affinity purification is commonly used to isolate and purify glutathione S-transferase (GST)-tagged fusion proteins from total cellular...
Autores principales: | Buhrman, Jason S, Rayahin, Jamie E, Köllmer, Melanie, Gemeinhart, Richard A |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463477/ https://www.ncbi.nlm.nih.gov/pubmed/22989306 http://dx.doi.org/10.1186/1472-6750-12-63 |
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