Determining Biophysical Protein Stability in Lysates by a Fast Proteolysis Assay, FASTpp

The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp). Combining unfolding by a temperature gradient in a thermal cycler with simultaneo...

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Detalles Bibliográficos
Autores principales: Minde, David P., Maurice, Madelon M., Rüdiger, Stefan G. D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463568/
https://www.ncbi.nlm.nih.gov/pubmed/23056252
http://dx.doi.org/10.1371/journal.pone.0046147

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463568/
https://www.ncbi.nlm.nih.gov/pubmed/23056252
http://dx.doi.org/10.1371/journal.pone.0046147

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