Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles

BACKGROUND: Temperature-sensitive (Ts) plasmids are useful tools for genetic engineering, but there are currently none compatible with the gram positive, thermophilic, obligate anaerobe, Clostridium thermocellum. Traditional mutagenesis techniques yield Ts mutants at a low frequency, and therefore r...

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Detalles Bibliográficos
Autores principales: Olson, Daniel G, Lynd, Lee R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3464808/
https://www.ncbi.nlm.nih.gov/pubmed/22578246
http://dx.doi.org/10.1186/1754-1611-6-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3464808/
https://www.ncbi.nlm.nih.gov/pubmed/22578246
http://dx.doi.org/10.1186/1754-1611-6-5

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