Gram Stains: A Resource for Retrospective Analysis of Bacterial Pathogens in Clinical Studies

We demonstrate the feasibility of using qPCR on DNA extracted from vaginal Gram stain slides to estimate the presence and relative abundance of specific bacterial pathogens. We first tested Gram stained slides spiked with a mix of 10(8) cfu/ml of Escherichia coli and 10(5) cfu/ml of Lactobacillus ac...

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Detalles Bibliográficos
Autores principales: Srinivasan, Usha, Ponnaluri, Sreelatha, Villareal, Lisa, Gillespie, Brenda, Wen, Ai, Miles, Arianna, Bucholz, Brigette, Marrs, Carl F., Iyer, Ram K., Misra, Dawn, Foxman, Betsy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469605/
https://www.ncbi.nlm.nih.gov/pubmed/23071487
http://dx.doi.org/10.1371/journal.pone.0042898

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469605/
https://www.ncbi.nlm.nih.gov/pubmed/23071487
http://dx.doi.org/10.1371/journal.pone.0042898

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