Construction of a Stability Landscape of the CH3 Domain of Human IgG1 by Combining Directed Evolution with High Throughput Sequencing

One of the most important but still poorly understood issues in protein chemistry is the relationship between sequence and stability of proteins. Here, we present a method for analyzing the influence of each individual residue on the foldability and stability of an entire protein. A randomly mutated...

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Detalles Bibliográficos
Autores principales: Traxlmayr, Michael W., Hasenhindl, Christoph, Hackl, Matthias, Stadlmayr, Gerhard, Rybka, Jakub D., Borth, Nicole, Grillari, Johannes, Rüker, Florian, Obinger, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469823/
https://www.ncbi.nlm.nih.gov/pubmed/22846908
http://dx.doi.org/10.1016/j.jmb.2012.07.017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469823/
https://www.ncbi.nlm.nih.gov/pubmed/22846908
http://dx.doi.org/10.1016/j.jmb.2012.07.017

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